cxcl16 recombinant protein  (MedChemExpress)

Journal: Clinical Cancer Research

Article Title: Redirecting B7-H3.CAR T Cells to Chemokines Expressed in Osteosarcoma Enhances Homing and Antitumor Activity in Preclinical Models

doi: 10.1158/1078-0432.CCR-23-3298

Figure Lengend Snippet: RNA-seq and multiplex analyses identify chemokines produced by primary OS samples. A, Expression of 28 chemokines by RNA-seq (FPKM shown; n = 85 OS samples); screen cutoff shown at 1 FPKM. B, Expression of 28 chemokines by protein secretion ( n = 8 patient samples); screen cutoff shown at 3 × 10 3 integrated pixel density. Green bars in A and B denote ligands deemed positive in both RNA and protein screens; error bars indicate SEM. C, Chemokine receptors (ligands in parentheses) CXCR2 (IL8), CXCR3 (CXCL10), CXCR6 (CXCL16), CCR7 (CCL21), and CCR8 (CCL18) as expressed endogenously and detected by flow cytometry on activated T cells ( n = 5 healthy donors; error bars indicate SEM). D, ELISA demonstrating detection of IL8 and CXCL16 in patient transport media specimens ( n = 6 patient tumor samples, 1 normal bone sample; error bars indicate SEM).

Article Snippet: At time point 0, the transwell was placed into a fresh V-shaped lower-chamber plate with 100 μL of RPMI supplemented with 1% FBS and 0, 10, or 30 ng/mL of recombinant human IL8 (R&D Systems) or recombinant human CXCL16 (R&D Systems).

Techniques: RNA Sequencing Assay, Multiplex Assay, Produced, Expressing, Flow Cytometry, Enzyme-linked Immunosorbent Assay

Journal: Nature Communications

Article Title: ITGB6 modulates resistance to anti-CD276 therapy in head and neck cancer by promoting PF4 + macrophage infiltration

doi: 10.1038/s41467-024-51096-0

Figure Lengend Snippet: A Representative flow plots show the frequency of the CXCR6 + CD8 + GZMB + T cells (left) and quantification of the proportions of CXCR6 + CD8 + GZMB + T cells (right) in groups with different PF4 + Macrophage and CD8 + T-cell proportions. Data are shown as mean ± SD (n = 3 biological replicates). P values are presented by one-way ANOVA with Tukey’s multiple comparison test. B Representative flow plots show the frequency of the CXCR6 + CD8 + PDCD1 + T cells (left) and quantification of the proportions of CXCR6 + CD8 + PDCD1 + T cells (right) in groups with different PF4 + Macrophage and CD8 + T-cell proportions. Data are shown as mean ± SD (n = 3 biological replicates). P values are presented by one-way ANOVA with Tukey’s multiple comparison test. C , D Representative flow plots show frequency of the CXCR6 + CD8 + GZMB + T cells ( C ) and quantification of the proportions of CXCR6 + CD8 + GZMB + T cells ( D ) in groups with different concentrations of CXCL16 recombinant protein. Data are shown as mean ± SD (n = 3 biological replicates). P values are presented by one-way ANOVA with Tukey’s multiple comparison test. E , F Representative flow plots show the frequency of the CXCR6 + CD8 + PDCD1 + T cells ( E ) and quantification of the proportions of CXCR6 + CD8 + PDCD1 + T cells ( F ) in groups with different concentrations of CXCL16 recombinant protein. Data are shown as mean ± SD (n = 3 biological replicates). P values are presented by one-way ANOVA with Tukey’s multiple comparison test. G Representative images of 4NQO-induced HNSCC (upper) and corresponding HE staining(lower) in groups with different concentrations of CXCL16 recombinant protein. Scale bar, 1 mm (upper), 100 μm (lower). H – K Representative images of KI67 ( H ) and Caspase-3 ( J ) IHC staining and quantitation of the percentage of KI67 + ( I ) and Caspase-3 + ( K ) cells in groups with different concentrations of CXCL16 recombinant protein. Scale bar, 50 μm. Data are shown as mean ± SD (n = 8 mice). P values are presented by one-way ANOVA with Tukey’s multiple comparison test. Source data and exact p values are provided as a Source Data file.

Article Snippet: In the co-culture system, the Moc1 cells were treated with different proportions of CD8 + T cells and PF4 + Macrophages, or with different concentrations of CXCL16 recombinant protein (HY-P700266, MCE).

Techniques: Comparison, Recombinant, Staining, Immunohistochemistry, Quantitation Assay

Journal: PLOS Pathogens

Article Title: The CXCR6-CXCL16 axis mediates T cell control of polyomavirus infection in the kidney

doi: 10.1371/journal.ppat.1012969

Figure Lengend Snippet: (A) CXCL16 mRNA expression in the kidney during MuPyV infection. Expression is shown as fold change relative to sham infected samples. Data are from three independent experiments (n = 10-11). (B) Expression of NKCC2 [marks the ascending loop of Henle ], CD8, and CXCL16 in epithelia in sham-infected and 8 dpi kidneys; bottom right photomicrographs are merged images. Representative of two independent experiments. (C) CXCL16 expression in sham-infected, 4 dpi, and 8 dpi kidney lysates (left). Western blot image is representative of two independent experiments with each lane indicating protein lysate from kidneys of individual mice. Protein band intensity quantification for sCXCL16 was normalized to β-actin and analyzed by ImageLab and normalized to the loading control (right). Data are combined from two independent experiments (n = 3-5). (D) Experimental design of in vivo CXCL16 mAb administration. Mice were administered 250 µg of CXCL16 mAb or control rat IgG every two days from days 4-14 post infection and euthanized at 15 dpi. Numbers of CD45 mAb i.v.-negative, CD8 + CD44 + D b -LT359 tetramer + T cells and CD4 + CD44 + T cells in kidneys and spleens of infected mice given anti-CXCL16 or control rat IgG. Data were analyzed by one-way ANOVA (A and C) and by multiple Mann-Whitney tests (D).

Article Snippet: For CXCL16 neutralization, mice were infected with MuPyV and received 250 μg of anti-mouse CXCL16 mAb (clone 12-81, BioXCell) i.p. on alternate days from days 4-14 post infection or control rat IgG (Jackson ImmunoResearch Laboratories).

Techniques: Expressing, Infection, Western Blot, Control, In Vivo, MANN-WHITNEY

Journal: PLOS Pathogens

Article Title: The CXCR6-CXCL16 axis mediates T cell control of polyomavirus infection in the kidney

doi: 10.1371/journal.ppat.1012969

Figure Lengend Snippet: (A) Dense lymphoplasmacytic infiltrate surrounding and infiltrating cortical tubules (H&E, x400). (B) Immunohistochemical staining for Large T antigen in nuclei of tubular epithelium (x250). (C) Expression of CXCR6 on infiltrating CD8 + cells. Photomicrographs (right) are enlarged images in the white squares (left); bottom right are merged images. No 1 o , no primary antibody. (D) Log-fold change of CXCL16 and CXCR6 across four independent studies, each comparing KTx biopsies from patients with PVAN and stable graft function. The error bars indicate the 95% confidence interval of log-fold change, with horizontal dashed grey line indicating log-fold change = 0, or no difference. GSE120495 is an RNA-seq study, while remaining studies are microarray based. Given the heterogeneity of the data, a Cauchy combination method with uniform weights was employed to integrate the p-values across these studies. The combined p-values for CXCL16 = 0.000337 and CXCR6 = 2.72x10 -05 . ns, non-significant at adjusted p-value > 0.05; *, statistical significance at adjusted p-value ≤ 0.05; **, statistical significance at adjusted p-value ≤ 0.01; ***, statistical significance at adjusted p-value ≤ 0.001.

Article Snippet: For CXCL16 neutralization, mice were infected with MuPyV and received 250 μg of anti-mouse CXCL16 mAb (clone 12-81, BioXCell) i.p. on alternate days from days 4-14 post infection or control rat IgG (Jackson ImmunoResearch Laboratories).

Techniques: Immunohistochemical staining, Staining, Expressing, RNA Sequencing, Microarray

Journal: PLOS Pathogens

Article Title: The CXCR6-CXCL16 axis mediates T cell control of polyomavirus infection in the kidney

doi: 10.1371/journal.ppat.1012969

Figure Lengend Snippet: Summary of differential gene expression of CXCL16 and CXCR6 in four studies of KTx biopsies with PVAN. Because of the heterogeneity of the data (i.e., one RNA-seq, three microarray), a Cauchy combination method with uniform weights was employed to integrate the p-values across these studies. P-values significant after false discover rate (FDR) correction are bolded and italicized in the Adj. P-value column. Cauchy combined p-values < 0.05 are bolded and italicized.

Article Snippet: For CXCL16 neutralization, mice were infected with MuPyV and received 250 μg of anti-mouse CXCL16 mAb (clone 12-81, BioXCell) i.p. on alternate days from days 4-14 post infection or control rat IgG (Jackson ImmunoResearch Laboratories).

Techniques: Gene Expression, Microarray

Journal: PLOS Pathogens

Article Title: The CXCR6-CXCL16 axis mediates T cell control of polyomavirus infection in the kidney

doi: 10.1371/journal.ppat.1012969

Figure Lengend Snippet: (A) CXCL16 mRNA expression in the kidney during MuPyV infection. Expression is shown as fold change relative to sham infected samples. Data are from three independent experiments (n = 10-11). (B) Expression of NKCC2 [marks the ascending loop of Henle ], CD8, and CXCL16 in epithelia in sham-infected and 8 dpi kidneys; bottom right photomicrographs are merged images. Representative of two independent experiments. (C) CXCL16 expression in sham-infected, 4 dpi, and 8 dpi kidney lysates (left). Western blot image is representative of two independent experiments with each lane indicating protein lysate from kidneys of individual mice. Protein band intensity quantification for sCXCL16 was normalized to β-actin and analyzed by ImageLab and normalized to the loading control (right). Data are combined from two independent experiments (n = 3-5). (D) Experimental design of in vivo CXCL16 mAb administration. Mice were administered 250 µg of CXCL16 mAb or control rat IgG every two days from days 4-14 post infection and euthanized at 15 dpi. Numbers of CD45 mAb i.v.-negative, CD8 + CD44 + D b -LT359 tetramer + T cells and CD4 + CD44 + T cells in kidneys and spleens of infected mice given anti-CXCL16 or control rat IgG. Data were analyzed by one-way ANOVA (A and C) and by multiple Mann-Whitney tests (D).

Article Snippet: For CXCL16 neutralization, mice were infected with MuPyV and received 250 μg of anti-mouse CXCL16 mAb (clone 12-81, BioXCell) i.p. on alternate days from days 4-14 post infection or control rat IgG (Jackson ImmunoResearch Laboratories).

Techniques: Expressing, Infection, Western Blot, Control, In Vivo, MANN-WHITNEY

Journal: PLOS Pathogens

Article Title: The CXCR6-CXCL16 axis mediates T cell control of polyomavirus infection in the kidney

doi: 10.1371/journal.ppat.1012969

Figure Lengend Snippet: (A) Dense lymphoplasmacytic infiltrate surrounding and infiltrating cortical tubules (H&E, x400). (B) Immunohistochemical staining for Large T antigen in nuclei of tubular epithelium (x250). (C) Expression of CXCR6 on infiltrating CD8 + cells. Photomicrographs (right) are enlarged images in the white squares (left); bottom right are merged images. No 1 o , no primary antibody. (D) Log-fold change of CXCL16 and CXCR6 across four independent studies, each comparing KTx biopsies from patients with PVAN and stable graft function. The error bars indicate the 95% confidence interval of log-fold change, with horizontal dashed grey line indicating log-fold change = 0, or no difference. GSE120495 is an RNA-seq study, while remaining studies are microarray based. Given the heterogeneity of the data, a Cauchy combination method with uniform weights was employed to integrate the p-values across these studies. The combined p-values for CXCL16 = 0.000337 and CXCR6 = 2.72x10 -05 . ns, non-significant at adjusted p-value > 0.05; *, statistical significance at adjusted p-value ≤ 0.05; **, statistical significance at adjusted p-value ≤ 0.01; ***, statistical significance at adjusted p-value ≤ 0.001.

Article Snippet: For CXCL16 neutralization, mice were infected with MuPyV and received 250 μg of anti-mouse CXCL16 mAb (clone 12-81, BioXCell) i.p. on alternate days from days 4-14 post infection or control rat IgG (Jackson ImmunoResearch Laboratories).

Techniques: Immunohistochemical staining, Staining, Expressing, RNA Sequencing, Microarray

Journal: PLOS Pathogens

Article Title: The CXCR6-CXCL16 axis mediates T cell control of polyomavirus infection in the kidney

doi: 10.1371/journal.ppat.1012969

Figure Lengend Snippet: Summary of differential gene expression of CXCL16 and CXCR6 in four studies of KTx biopsies with PVAN. Because of the heterogeneity of the data (i.e., one RNA-seq, three microarray), a Cauchy combination method with uniform weights was employed to integrate the p-values across these studies. P-values significant after false discover rate (FDR) correction are bolded and italicized in the Adj. P-value column. Cauchy combined p-values < 0.05 are bolded and italicized.

Article Snippet: For CXCL16 neutralization, mice were infected with MuPyV and received 250 μg of anti-mouse CXCL16 mAb (clone 12-81, BioXCell) i.p. on alternate days from days 4-14 post infection or control rat IgG (Jackson ImmunoResearch Laboratories).

Techniques: Gene Expression, Microarray

Journal: Oncoimmunology

Article Title: ELK3-CXCL16 axis determines natural killer cell cytotoxicity via the chemotactic activity of CXCL16 in triple negative breast cancer

doi: 10.1080/2162402X.2023.2190671

Figure Lengend Snippet: ELK3 depletion increases CXCL16 expression in TNBC cells.

Article Snippet: Recombinant human CXCL16 protein was purchased from PEPROTECH (300–55, Cranbury, NJ, USA).

Techniques: Expressing

Journal: Oncoimmunology

Article Title: ELK3-CXCL16 axis determines natural killer cell cytotoxicity via the chemotactic activity of CXCL16 in triple negative breast cancer

doi: 10.1080/2162402X.2023.2190671

Figure Lengend Snippet: The chemotactic activity of CXCL16 recruits NK cells to target TNBC cells.

Article Snippet: Recombinant human CXCL16 protein was purchased from PEPROTECH (300–55, Cranbury, NJ, USA).

Techniques: Activity Assay

Journal: Oncoimmunology

Article Title: ELK3-CXCL16 axis determines natural killer cell cytotoxicity via the chemotactic activity of CXCL16 in triple negative breast cancer

doi: 10.1080/2162402X.2023.2190671

Figure Lengend Snippet: The ELK3-CXCL16 axis determines the response of NK cells in TNBC cells.

Article Snippet: Recombinant human CXCL16 protein was purchased from PEPROTECH (300–55, Cranbury, NJ, USA).

Techniques:

Journal: Oncoimmunology

Article Title: ELK3-CXCL16 axis determines natural killer cell cytotoxicity via the chemotactic activity of CXCL16 in triple negative breast cancer

doi: 10.1080/2162402X.2023.2190671

Figure Lengend Snippet: CXCL16 promotes NK cell chemotaxis and cytotoxicity in vivo .

Article Snippet: Recombinant human CXCL16 protein was purchased from PEPROTECH (300–55, Cranbury, NJ, USA).

Techniques: Chemotaxis Assay, In Vivo

Journal: Oncoimmunology

Article Title: ELK3-CXCL16 axis determines natural killer cell cytotoxicity via the chemotactic activity of CXCL16 in triple negative breast cancer

doi: 10.1080/2162402X.2023.2190671

Figure Lengend Snippet: Negative correlation between ELK3 and CXCL16 in human breast cancer.

Article Snippet: Recombinant human CXCL16 protein was purchased from PEPROTECH (300–55, Cranbury, NJ, USA).

Techniques: